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Global riactice Leader's in Brain Science

6/4 (Mon) 16:00 Regular Seminar
Name : 관리자 | Date : 2018.05.30 13:43 | Views : 11649

Title: The C-Domain of the NAC Transcription Factor ANAC019 is Necessary for pH-Tuned DNA Binding through a Histidine Switch in the N-Domain


Speaker: Iksoo Chang, Ph.D., 
           Core Protein Resources Center, Supercomputing and Bigdata Center, Department of Brain and Cognitive Sciences, DGIST


Time: 16:00, June 4 (Mon), 2018
        

Venue: Room 114, Building E4, DGIST


Abstract : The affinity of transcription factors (TFs) for their target DNA is a critical determinant of gene expression. Whether DNA binding domain (DBD) of TFs alone can regulate a binding affinity to DNA is an important question for identifying the design principle of TFs. We studied ANAC019, a member of NAC TF family of proteins in Arabidopsis, and found a well-conserved histidine-switch located in its DBD, which regulates both homo-dimerization and transcriptional control of the TF through H135-protonation. Here, we found that the removal of C-terminal intrinsically disordered domain in the TF abolished the pH-dependent binding of N-terminal DBD to its target DNA. We propose its underlying mechanism that the DBD alone is too strongly attracted to DNA and has too long of a residence time near DNA to have the pH-dependency. However, its full-length, which possesses an electric dipole moment, could balance the strong attraction of the DBD to DNA.

 


Person in charge : Sora Lee


Contact: srlee@dgist.ac.kr, 053)785-6102

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