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Global riactice Leader's in Brain Science

6/28 (Fri) 14:00 Special Seminar
Name : 관리자 | Date : 2019.06.10 14:40 | Views : 45

Title: Genome-wide CRISPR screens in lung cancer spheroids identify 3D growth specific cancer vulnerabilities

Speaker: Kyuho Han, Ph.D. (Postdoctoral Scholar, Stanford University, Stanford, CA, USA) (Host : Jaewon Ko)


Time: 14:00, June 28 (Fri), 2019
        

Venue: Room 506, Building E4, DGIST


Abstract :Cancer is defined as uncontrolled 3D growth of cells driven by multiple mutations. Although numerous cancer genomics studies have revealed candidate drivers, it remains challenging to assign causality across diverse cancers. Here we devised a highly scalable cancer spheroid model and performed genome-wide CRISPR screens both in 2D- and 3D-growing lung cancers with G12C mutation. We found CRISPR-phenotypes of 3D accurately recapitulate those of in vivo tumor. Importantly, hits that show differential sensitivities between 2D and 3D are most strongly enriched with significant mutations in lung cancers. Further analysis on enriched co-essential interactomes in the top hits identified many known and novel candidate drivers for cancer 3D growth. Importantly, these newly identified drivers include a novel gene that is responsible for maturation of IGF1R, totally unreported process which found to be critical for the maximal receptor activity. We thus provide a novel strategy that exploits CRISPR in cancer spheroids to systematically and scalably investigate cancer vulnerabilities for targeted therapeutics. 

 

Person in charge : BoGyu Jang

 

Contact: jangbk87@dgist.ac.kr, 053)785-6101

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