DIGST Department of Brain Sciences
To establish a core facility for the Department of Brain Science that will support up to 50 Principal Investigators along with their post-doctoral candidates and students.
To provide instrumentation covering all microscopy and imaging needs for immediate applications with allowance for future growth within the Institute.
To concentrate on biological sciences with functional solutions that will produce direct results.
|High resolution confocal microscope (Carl Zeiss/LSM 700)||Vivatome microscope (Carl Zeiss/Axio Observer Z1&VivaTome)||Up-right fluorescence microscope (Nikon/eclipse 90i)|
|Stereo Microscope for Fluorescence (Leica/M205FA)||Live cell confocal microscope (Carl Zeiss/LSM 7 Live)||Computer workstations for image analysis and data processing|
|Histology solutions||High resolution confocal microscope (Carl Zeiss/LSM 800)||Slide scan system model AXIO SCAN Z1|
Laboratory Animal Facility is a component of campus animal resources that provides care for laboratory animals for investigators in the department of Brain Science at DGIST. It is dedicated to the humane use of animals in research and teaching.
The Institutional Animal Care and Use Committee (IACUC) - The IACUC reviews protocols, and the animal care and use program, and monitors related activities to ensure compliance with standards and regulatory requirements. The IACUC includes scientists, veterinarians, and administrative staffs.
Animal housing rooms - It has capacity to house the laboratory mouse and rats in a separate room.
Services are provided on a fee-for-service basis and detailed for investigators in a monthly billing in a manner that meets grant and contractual administrative standards. Brain Science is committed to a certified program for the care of laboratory animals used in biomedical research and testing. The facility provides for separation of species, separation/containment for experimental conditions in a bio-containment environment, and designated space for various functions. Specifically, the facility includes the following:
The Biochemistry Core Lab provides investigators with a wide range of biochemical laboratory support and access to a well-equipped research laboratory suite. Carefully maintained quality control systems are in place for each assay or analysis. In addition to the methods and technologies currently in place, new techniques and innovative methods may be incorporated by the Brain Science equipment committee to meet the specific needs of your protocol. The Biochemistry Core lab assists investigators in coordinating aspects of obtaining research samples in an efficient, accurate manner as well as aiding investigators in fulfilling unique laboratory requirements dictated by their protocol.
|CO2 incubator||Centrifuge||ChemiDoc||Dry Autoclave|
|Eclips 90i||FACS||Fume Hood||GelDoc EZ Imaging system|
|Hydridzation incubator||Hot plate Stirrer||ICE Maker||LSM700|
|LSM780||LSM 7 LIVE||LN2 Supply Tank||Luminometer|
|Milli-Q Advangage A10||Neurolucida360||NANODROP 2000 Spectrophotometer||Optima XPN-100 Ultracentrifuge|
|Optima MAX-XP||pH meter||Real-Time System||Refregerated Micro centrifuge|
|SRX-201A||Shaking incubator||Sonicator||SpeedVac Concentrator|
|Transilluminator box||UV/visible Spectrophotometer||Ultra Low Temperature Freezer||VERSA max|
The Tissue and Cell Culture Core Laboratory provides active culture systems for many types of mammalian cell lines and primary tissues, as well as maintains frozen stocks of a larger cell library.
General tasks include maintenance of active eukaryotic cell cultures and plating cells for experimental procedures. The Core has two fluorescence microscopes for detecting cells expressing any fluorescent indicators and picturing the image of cells using camera system. Other approaches available include cell cloning, establishment of primary cell cultures including mouse neuronal and glial cells, mycoplasma testing, specialized media preparation and generation of stable cell lines.
|Biological safety cabinet||Water jacketed CO2 incubator||Tissue culture microscope (Cal Zeiss Axiovert 40)|
|Refrigerated centrifuge||Freezer and refrigerator||Water bath|
The Histology Core Laboratory provides comprehensive histology services for all rodent species (mouse, rat, etc.). General tasks include tissue preparation, embedding, processing, frozen and paraffin sectioning, and histochemical staining of primarily rodent samples. In addition, the Core Lab provides training opportunities for learning histology techniques and phenotyping analysis. The Core Lab possesses Tissue Processor, Tissue Embedding Console System, Microtome and other analytical equipment.
|Dry oven||Tissue Processor||Embedding Console System||Microtome|