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1/28 (Mon) 16:00 Special Seminar
Name : 관리자 | Date : 2019.01.24 16:47 | Views : 896

Title: Single-cell and single-molecule transcriptome profiling with RNA modifications


Speaker: Kyung Lock Kim, Ph.D., Massachusetts General Hospital, Harvard Medical School, and The Broad Institute (Host : Han Kyoung Choe)


Time: 16:00, January 28 (Mon), 2019
        

Venue: Room 406, Building E4, DGIST


Abstract : An extensive repertoire of epigenetic modifications is known to influence transcriptional and post-transcriptional steps in gene expression by controlling the versatile coding, structural and catalytical functions. Although diverse bulk sequencing techniques with epigenetic modifications are developed, in-depth study of single-cell epigenetic sequencing (DNA, RNA and histone modifications) has been impeded by a lack of robust analytical methods. Here we present the novel single-cell sequencing method to profile transcriptome with RNA modifications. Using >50k nanowell technique for single-cell isolation and high-throughput single-molecule imaging, we reveal that individual cells exhibit different level of each epigenetic mark in single-cell transcriptome. In addition, we demonstrate the single-molecule sequencing with combinatorial epigenetic marks in individual cells. Finally, we identify cell subpopulations defined by differences in epi-transcriptomic states. Our approach has the potential to uncover combinatorial modifications states on single molecules, and to investigate their variability across single cells and conditions.


Person in charge : Sora Lee


Contact: srlee@dgist.ac.kr, 053)785-6102

IP : 10.140.1.*** share
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No File Subject Name Date Views
png 관리자 2019.02.19 4,248
125 pdf 관리자 2019.01.25 2,510
124 pdf 관리자 2019.01.24 963
123 pdf 관리자 2019.01.24 893
pdf 관리자 2019.01.24 897
121 pdf 관리자 2018.12.06 1,077
120 pdf 관리자 2018.11.20 1,034
119 pdf 관리자 2018.11.15 1,105
118 pdf 관리자 2018.11.15 1,098
117 pdf 관리자 2018.10.15 1,267
116 pdf 관리자 2018.10.11 1,231
115 pdf 관리자 2018.10.11 1,488
114 pdf 관리자 2018.10.10 1,530
113 pdf 관리자 2018.10.02 1,580
112 pdf 관리자 2018.09.10 1,691
111 pdf 관리자 2018.09.10 1,601
110 pdf 관리자 2018.08.29 1,775
109 jpg 관리자 2018.08.20 5,789
108 pdf 관리자 2018.07.26 5,321
107 pdf 관리자 2018.07.16 5,242
106 pdf 관리자 2018.06.21 6,128