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1/28 (Mon) 16:00 Special Seminar
Name : 관리자 | Date : 2019.01.24 16:47 | Views : 725

Title: Single-cell and single-molecule transcriptome profiling with RNA modifications


Speaker: Kyung Lock Kim, Ph.D., Massachusetts General Hospital, Harvard Medical School, and The Broad Institute (Host : Han Kyoung Choe)


Time: 16:00, January 28 (Mon), 2019
        

Venue: Room 406, Building E4, DGIST


Abstract : An extensive repertoire of epigenetic modifications is known to influence transcriptional and post-transcriptional steps in gene expression by controlling the versatile coding, structural and catalytical functions. Although diverse bulk sequencing techniques with epigenetic modifications are developed, in-depth study of single-cell epigenetic sequencing (DNA, RNA and histone modifications) has been impeded by a lack of robust analytical methods. Here we present the novel single-cell sequencing method to profile transcriptome with RNA modifications. Using >50k nanowell technique for single-cell isolation and high-throughput single-molecule imaging, we reveal that individual cells exhibit different level of each epigenetic mark in single-cell transcriptome. In addition, we demonstrate the single-molecule sequencing with combinatorial epigenetic marks in individual cells. Finally, we identify cell subpopulations defined by differences in epi-transcriptomic states. Our approach has the potential to uncover combinatorial modifications states on single molecules, and to investigate their variability across single cells and conditions.


Person in charge : Sora Lee


Contact: srlee@dgist.ac.kr, 053)785-6102

IP : 10.140.1.*** share
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png 관리자 2019.02.19 3,589
125 pdf 관리자 2019.01.25 1,654
124 pdf 관리자 2019.01.24 793
123 pdf 관리자 2019.01.24 711
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121 pdf 관리자 2018.12.06 892
120 pdf 관리자 2018.11.20 857
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118 pdf 관리자 2018.11.15 903
117 pdf 관리자 2018.10.15 1,079
116 pdf 관리자 2018.10.11 1,050
115 pdf 관리자 2018.10.11 1,253
114 pdf 관리자 2018.10.10 1,328
113 pdf 관리자 2018.10.02 1,368
112 pdf 관리자 2018.09.10 1,483
111 pdf 관리자 2018.09.10 1,391
110 pdf 관리자 2018.08.29 1,571
109 jpg 관리자 2018.08.20 5,316
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